Asculine is a glycoside and it is incorporated with a nutrient base a ferric salt. Aesculin hydrolysis means the breakdown of aesculin. This hydrolysis is indicated by a BROWN coloration.
It occurs due to the reaction of aglycone (6.7 Dihydroxycoumarin) with iron. Sometimes another chemical (SODIUM AZIDE) is added. It acts as a preservative.
The Aesculin hydrolysis test is used to identify the enterococci, anaerobic cocci, streptococci, and others.
Some organism hydrolyse aesculine. It results in the formation of a substance called AGLYCONE. It reacts with iron and forms a compound that is BROWN to BLACK in color.
Following are the steps in the aesculin test procedure.
All other streptococci
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