Ion selective electrode principle, procedure (ISE), types, diagram

ion selective electrode principle procedure ESE types diagram

Ion-selective electrode (ISE) is an important instrument for analyzing various electrolytes like Sodium, Potassium, Chloride, and Lithium in the pathology laboratory. ISE is used in many departments of the medical field. Principle of the ion selective electrode (ISE) ISE principle is that, it create potential that is proportional to the concentration of the tested analyte. … Read more

Heat fixation procedure, purpose ,factors affecting fixation microbiology

Heat fixation procedure, purpose ,factors affecting fixation microbiology

Heat fixation is the second method after the chemical fixation in histology.  what is heat fixation in microbiology? The mechanism of heat fixation is, that it denatures the protein. It takes two to three minutes.  Microwave ovens have overtaken the conventional heating method, due to their controlled environment. 45-55 C is the optimum temperature of … Read more

10% neutral buffered formalin composition

10% Neutral Buffered Formalin is widely used in labs for preserving biological tissues. It stabilizes tissue structures by cross-linking proteins, and the buffer system ensures a neutral pH, which helps reduce tissue damage during fixation. Here’s the composition of 1 liter of 10% Neutral Buffered Formalin: How to prepare 1 liter 10% neutral buffered formalin … Read more

Tissue fixation types mechanism( bone, skin, biopsies, liver) histology

It is fixation is important in tissue processing because it preserves the anatomy of different tissues. It makes tissue hard so that a thin section can be made from it.   Tissue fixation means the process by which components of the cell and tissues are fixed physically and partially by chemical methods. So that it … Read more

Cytology cell block preparation procedure steps cytology

A cytology cell block is an important procedure. Block can be prepared from the FNA aspirant or other body fluids like peritoneal, and cerebrospinal fluid. Cell block preparation procedure steps in the cytology The following are the cell block preparation steps in the cytology lab. The aspirated material from the syringe is rinsed thoroughly with … Read more

Fine needle aspiration biopsy procedure thyroid cytology (fnac), complications

Fine needle aspiration procedure steps cytology (fnac), complications

Fine needle aspiration cytology procedure or FNAc. It is an inexpensive but important technique in the cytology laboratory. It is a rapid method of assessing the lesions and masses in the body. Fine needle aspiration is a type of aspiration by which a fine needle is inserted and used to collect the sample from superficial … Read more

Cytology specimen collection and handling (Sputum specimen) in histology lab

There are many types of specimens for cytological examination. Cytology specimen collection and handling are very sensitive. Each sample has its own standard operating procedure. But some steps are common in all.  We need these things     Fixatives Container or jar – Coplin jar made of glass or plastic is used as a fixative … Read more

Clinical microbiology final exam quizlet Chapter 5-6

Here is the most important Quizlet of the microbiology final exam for medical students to refresh their knowledge. Chose the gram-positive bacteria. a. Neisseria, b. Klebsiella, c. Clostridium Free-living, Thick wall, cause abscess of skin. Characteristics of.  a. Streptococcus b. Staphylococcus c.  Bacillus Extracellular bacteria, thick wall, spore-forming aerobe Characteristics of. a. Clostridium b. Corynebacterium … Read more

C-reactive protein test method procedure, High, Low

c-reactive protein blood test principle, procedure, indications, High, Low

CRP test is an important biomarker of inflammatory cytokinesis such as interleukin-6. Therefore c-reactive blood test is used to assess their concentration. It is classified as an acute-phase reactant, which means c-reactive protein concentration rises within a few hours. C-reactive protein increases in the following conditions, Cell injuries Infections Inflammation C-reactive protein principle This test … Read more

Tissue collection method in histology laboratory (Liver, bone, renal)

We collect and deal with different types of tissues biopsy in the histopathology laboratory. The tissue collection method in the histology laboratory has the following steps. Role of Fixative in tissue collection method After the removal of surgical specimens, they should be placed in a container filled with fixative. For the best fixation, tissue should … Read more