hematoxylin and eosin stain is commonly used stains in the histopathology and cytology section of the clinical laboratory. It is preferred for viewing cellular and tissue structure detail by pathologists. It demonstrates the broad range of cytoplasmic, nuclear, and extracellular matrix features of the tissue. Hematoxylin eosin stain Principle First of all, wax cleared tissues … Read more
Red blood cells (RBC) have a negative charge on their surface due to SIALIC ACID. This charge prevents the clumping of Red blood cells. Zeta Potential principle RBCs have a negative charge on their surface due to sialic acid. Therefore red blood cells repel the adjacent red blood cells (Having the same charge), result in … Read more
The sickling test, also known as the sickle cell test, is a laboratory test used to diagnose sickle cell disease, (a genetic blood disorder) . It involves taking a small sample of blood from the patient and examining it under a microscope to look for the presence of sickle-shaped red blood cells. Therefore Sickling test … Read more
The hematocrit is an important test for estimating the average volume of red blood cells. Anticoagulated blood is used for hematocrit tests. You can calculate mean corpuscular volume (MCV) with the help of HCT. Manual hematocrit calculation methods There are two methods for calculating the hematocrit. Wintrobe’s method of Hematocrit (macrohematocrit) test requirements, procedure , … Read more
The Bile solubility test is important for the identification of Streptococcus pneumoniae from Viridans types of other streptococci. Principle of bile Solubility test Some bacteria (Strept Pneumoniae) have an autolytic enzyme that autolyzes the broth culture within a few days. while other alpha-hemolytic bacteria are resistant to bile. Bile Solubility test reagents and media The … Read more
The indole test is a biochemical test used primarily in microbiology to determine an organism’s ability to produce indole from the amino acid tryptophan. It is particularly useful for identifying certain species of bacteria. Principle of indole test when some bacteria react with Tryptophan (amino acid), hydrolysis it and change it o Indole compound. This … Read more
Urease test is an important biochemical test for the identification of certain kinds of bacteria like helicobacter pylori, proteus, morganella, and enterocolitis. What is the principle of the urease test? Some bacteria produce enzymes called urease. When they react with urea, they split it and convert it into ammonia (NH3) and carbon dioxide (CO2). Ammonia … Read more
The deoxyribonuclease test (DNAse) is an important biochemical test for the conformation of staph aureus. Principle of Deoxyribonuclease test (DNAse) Staph aureus has an enzyme called DEOXYRIBONULEASE. When DNA (deoxyribose nucleic acid) reacts with staph aureus, its enzyme( DEOXYRIBONULEASE ) hydrolyzes the DNA. Which reagents are used for the DANse test? Basically, two reagents are … Read more
Oxidase test helps to identify the vibro, Neisseria and Pseudomonas. Principle of oxidase test Pseudomonas Aurogenosa produced Oxidase Enzyme, which oxidizes the Phenylene Diamine to purple-colored compound. Reagent for Oxidase test Oxidase reagent is freshly prepared. It is a 1% solution of Tetramethyl-p-Phenylene Diamine Dihydrochloride. Oxidase Test Procedure Following steps are performed for oxidase test … Read more
Here you will learn about the “Coagulase test principle procedure”. The main purpose of this test is to differentiate the staph aureus from other types of staph. Staph aureus contains a coagulase enzyme that coagulates plasma. Note: Coagulase is an enzyme that exists in one type of staph (Staph aureus). Therefore this test named as … Read more