Microbiology

Phenylalanine deaminase test principle, procedure, and purpose

The phenylalanine deaminase test is important to differentiate proteus and providendia from other enterobacteria and enterocolitis. Principle of phenylalanine deaminase test Some enterobacteria members can form phenyl pyruvic acid from phenylalanine by oxidative deamination. When this phenylalanine reacts with acidified ammonium sulfate OR 10 % ferric chloride solution to form phenyl pyruvic acid.This phenyl pyruvic …

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Arginine dihydrolase test procedure for pseudomonas aeruginosa

Arginine is an amino acid that builds up protein. Arginine is hydrolyzed by some bacteria, which release an enzyme called arginine dihydrolase. It is the characteristics of certain enterobacteria. Arginine dihydrolase test procedure There are two types of procedures. Procedure: 1 Following are the steps in procedure 1. Inoculate 5 ml arginine broth. Incubate for …

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