Bile Tolerance test is used to differentiate the streptococcus agalectiae from other gram positive cocci. Certain streptococci (S.agalactiae) and enterococci are resistant to 10-40% bile and this test is used to differentiate these from other Gram-positive cocci. Anaerobic bacteria also vary in their ability to grow in the presence of 20% bile. Bile tolerance is …
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Potassium cyanide medium used to grow the certain kinds of bacteria. Some bacteria grow in the presence of KCN. Potassium cyanide medium test Reagent (KCN test) Nutrient Broth Base : 1 litter Potassium Cyanide Solution 5% : 15 ml Dispense the medium in 1 ml quantities into sterile tubes and stopper quickly with corks. Sterilize …
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Methyl red test or MR test is an important biochemical test used to differentiate the enterobacteria. Methyl red is used as a PH indicator. Methyl red test principle Some bacteria create sufficient acidity from glucose to give RED color with methyl red. While other organisms do not produce more acidity from glucose. Therefore in the …
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CAMP test stands for Christie, Atkins, Munch-Peterson test in microbiology. A clear area around the blood agar plate is the sign of a positive CAMP test. CAMP test principle A positive CAMP test produces a clear area around the colony on the surface of a blood agar plate that has been affected by the staphylococcus …
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The litmus milk decolorization test is an important biochemical; test used to identify the clostridia. Litmus milk test principle A heavy inoculum of the test organism is incubated for up to 4 hours in a tube containing litmus milk. A change indicates a reduction of litmus milk in the medium color from mauve to white …
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The phenylalanine deaminase test is important to differentiate proteus and providendia from other enterobacteria and enterocolitis. Principle of phenylalanine deaminase test Some enterobacteria members can form phenyl pyruvic acid from phenylalanine by oxidative deamination. When this phenylalanine reacts with acidified ammonium sulfate OR 10 % ferric chloride solution to form phenyl pyruvic acid.This phenyl pyruvic …
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Arginine is an amino acid that builds up protein. Arginine is hydrolyzed by some bacteria, which release an enzyme called arginine dihydrolase. It is the characteristics of certain enterobacteria. Arginine dihydrolase test procedure There are two types of procedures. Procedure: 1 Following are the steps in procedure 1. Inoculate 5 ml arginine broth. Incubate for …
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