Clinical microbiology focuses on detecting, identifying, and managing microorganisms that cause diseases in humans. It plays a crucial role in diagnosing infections, monitoring outbreaks, and guiding treatments. Key areas include:
Clinical microbiology aids in developing targeted therapies, ensuring effective infection control, and improving public health.
The indole test is a biochemical test used primarily in microbiology to determine an organism’s ability to produce indole from the amino acid tryptophan. It is particularly useful for identifying certain species of bacteria. Principle of indole test when some bacteria react with Tryptophan (amino acid), hydrolysis it and change it o Indole compound. This … Read more
Urease test is an important biochemical test for the identification of certain kinds of bacteria like helicobacter pylori, proteus, morganella, and enterocolitis. What is the principle of the urease test? Some bacteria produce enzymes called urease. When they react with urea, they split it and convert it into ammonia (NH3) and carbon dioxide (CO2). Ammonia … Read more
The deoxyribonuclease test (DNAse) is an important biochemical test for the conformation of staph aureus. Principle of Deoxyribonuclease test (DNAse) Staph aureus has an enzyme called DEOXYRIBONULEASE. When DNA (deoxyribose nucleic acid) reacts with staph aureus, its enzyme( DEOXYRIBONULEASE ) hydrolyzes the DNA. Which reagents are used for the DANse test? Basically, two reagents are … Read more
Oxidase test helps to identify the vibro, Neisseria and Pseudomonas. Principle of oxidase test Pseudomonas Aurogenosa produced Oxidase Enzyme, which oxidizes the Phenylene Diamine to purple-colored compound. Reagent for Oxidase test Oxidase reagent is freshly prepared. It is a 1% solution of Tetramethyl-p-Phenylene Diamine Dihydrochloride. Oxidase Test Procedure Following steps are performed for oxidase test … Read more
Here you will learn about the “Coagulase test principle procedure”. The main purpose of this test is to differentiate the staph aureus from other types of staph. Staph aureus contains a coagulase enzyme that coagulates plasma. Note: Coagulase is an enzyme that exists in one type of staph (Staph aureus). Therefore this test named as … Read more
Albert staining is a kind of differential stain. It is used to stain the metachromatic granules in corynebacterium diphtheria. How do I make Albert stain? Here are the three reagents you need for Albert staining. Which mordant is used in Albert staining ? Albert indide is used as mordent. After Albert’s stain, slid covered with … Read more
Catalase test is important for identification of varius bacteria specially staphylococcus aureus in microbiology. Here we learn about principle of catalase test, procedure and the reagents. Catalase test principle microbiology Catalase synthesized by some bacteria acts as a catalyst in the breakdown of Hydrogen peroxide (3% H2O2) results in the production of Oxygen gas (O2) … Read more
AFB use carbol fuchsin (a red dye) followed by acid-alcohol decolorization and counterstaining with methylene blue. AFB appear bright red against a blue background. Modified afb stain procedure Following are the 4 steps in acid-fast staining. Why ziehl-neelsen stain (ZN staining) is called acid fast? In the ziehl Neilsen stain, we use strong acids such … Read more
Here is the gram staining procedure step-wise. Gram staining principle The gram staining technique is based on the variation in cell wall thickness. when a bacterial smear is mixed with a gram stain. Gram-positive bacteria retain crystal violet color due to their cell wall thickness. While iodine removes the violet stain on gram-negative bacteria (due … Read more
Hydatid cyst of the liver caused by the Cysticerci of a Cestode. Hydatid cyst caused by the Echinococcus granulosus. This disease is caused by cystic echinococcosis. Composite computer illustration showing Echinococcus granulosus hydatid cyst in the human liver and close-up view of larvae protoscolices inside hydatid cyst. E. granulosus is a parasitic tapeworm, also called … Read more