Here is the microbiology mcqs with answer from chapter 9.
- Which staining technique is commonly used to differentiate bacterial species into Gram-positive and Gram-negative groups?
a) Acid-fast staining
b) Capsule staining
c) Flagella staining
d) Gram staining Answer: d) Gram staining - The main purpose of throat culture is to detect the.
a) Staph Aureus
b) Strept pyogenes - What is the primary goal of PCR (Polymerase Chain Reaction) in microbiology diagnosis?
a) Identifying bacterial morphology
b) Amplifying and detecting specific DNA sequences
c) Measuring antibiotic susceptibility
d) Determining serological reactions Answer: b) Amplifying and detecting specific DNA sequences - Serological tests are used to detect:
a) Bacterial endospores
b) Viral RNA
c) Antibodies or antigens in a patient’s blood serum
d) Fungal cell walls Answer: c) Antibodies or antigens in a patient’s blood serum
- What is the main purpose of an antibiotic susceptibility test?
a) Identifying bacterial species
b) Determining the cause of an infection
c) Assessing the effectiveness of antibiotics against a specific microorganism
d) Detecting viral pathogens Answer: c) Assessing the effectiveness of antibiotics against a specific microorganism - If the venipuncture site is inadequately disinfected, a blood culture bottle is most often contaminated with which kind of bacteria?
a) E.coli
b) Staph epidermidis - Which laboratory technique allows for the identification of microorganisms based on their genetic material?
a) Gram staining
b) PCR (Polymerase Chain Reaction)
c) Serological testing
d) MALDI-TOF mass spectrometry Answer: b) PCR (Polymerase Chain Reaction) - What is the primary purpose of MALDI-TOF mass spectrometry in microbiology?
a) Amplifying DNA sequences
b) Identifying fungal spores
c) Analyzing protein profiles for bacterial and fungal identification
d) Determining bacterial motility Answer: c) Analyzing protein profiles for bacterial and fungal identification - Culture-independent diagnostic methods in microbiology are used primarily for:
a) Identifying bacteria based on colony morphology
b) Rapid growth of bacteria in liquid media
c) Detecting microorganisms without the need for culturing
d) Assessing antibiotic resistance Answer: c) Detecting microorganisms without the need for culturing - In a microbiology laboratory, what is the primary purpose of using biosafety cabinets?
a) Sterilizing laboratory equipment
b) Ensuring laboratory personnel wear PPE
c) Providing a controlled environment for handling microorganisms
d) Amplifying DNA sequences Answer: c) Providing a controlled environment for handling microorganisms - Which of the following is a safety measure to protect against biohazardous materials in a microbiology laboratory?
a) Wearing lab coats
b) Using glassware without gloves
c) Working in an open laboratory environment
d) Disposing of hazardous materials in regular waste bins Answer: a) Wearing lab coats
- Which of the following techniques involves the cultivation of microorganisms on selective media to encourage the growth of specific species?
a) Streak plating
b) Enrichment culturing
c) Gram staining
d) Acid-fast staining Answer: b) Enrichment culturing - In microbiology, the term “metagenomics” refers to:
a) Studying the metabolism of microorganisms
b) Identifying viruses using genetic markers
c) Analyzing the collective genetic material of microbial communities
d) Measuring the sensitivity of microorganisms to antibiotics Answer: c) Analyzing the collective genetic material of microbial communities - Which of the following is NOT a common application of molecular diagnostics in microbiology?
a) Identifying the genetic basis of antibiotic resistance
b) Detecting viral RNA in blood samples
c) Analyzing bacterial colony morphology
d) Investigating the genetic diversity of microbial populations Answer: c) Analyzing bacterial colony morphology - What is the primary purpose of the Kirby-Bauer disk diffusion method in microbiology?
a) Identifying bacterial species
b) Measuring bacterial motility
c) Assessing antibiotic susceptibility
d) Detecting fungal infections Answer: c) Assessing antibiotic susceptibility - Which of the following laboratory techniques is used to visualize the structural components of viruses?
a) Gram staining
b) Electron microscopy
c) MALDI-TOF mass spectrometry
d) PCR (Polymerase Chain Reaction) Answer: b) Electron microscopy - What type of microscope is commonly used for examining live microorganisms in a microbiology laboratory?
a) Scanning electron microscope (SEM)
b) Transmission electron microscope (TEM)
c) Phase-contrast microscope
d) Confocal microscope Answer: c) Phase-contrast microscope - In a microbiology laboratory, what does the term “aseptic technique” refer to?
a) The use of antimicrobial agents to sterilize equipment
b) Proper handling and manipulation of microorganisms to prevent contamination
c) The use of antibiotics to inhibit bacterial growth
d) Rapid culture growth under high-pressure conditions Answer: b) Proper handling and manipulation of microorganisms to prevent contamination - Which of the following is NOT a category of personal protective equipment (PPE) used in microbiology laboratories?
a) Lab coats
b) Safety goggles
c) Hairnets
d) Office attire Answer: d) Office attire - What is the primary advantage of using selective media in microbiology?
a) Promoting the growth of all microorganisms equally
b) Allowing the growth of only specific microorganisms
c) Enhancing the visibility of microbial colonies
d) Increasing the rate of antibiotic resistance Answer: b) Allowing the growth of only specific microorganisms - What is the main purpose of a biosafety level (BSL) designation in a microbiology laboratory?
a) To indicate the level of cleanliness in the laboratory
b) To specify the types of microorganisms that can be handled safely
c) To determine the color of laboratory equipment
d) To regulate laboratory funding Answer: b) To specify the types of microorganisms that can be handled safely - Which of the following staining techniques is commonly used to detect Mycobacterium tuberculosis in clinical samples?
a) Gram staining
b) Acid-fast staining
c) Flagella staining
d) Capsule staining Answer: b) Acid-fast staining - What is the purpose of using MacConkey agar in microbiology laboratories?
a) To promote the growth of Gram-positive bacteria
b) To identify fungal species
c) To differentiate lactose-fermenting from non-lactose-fermenting bacteria
d) To test antibiotic susceptibility Answer: c) To differentiate lactose-fermenting from non-lactose-fermenting bacteria - Which diagnostic method is most suitable for identifying the presence of viral RNA in a patient’s blood sample?
a) Gram staining
b) Serological testing
c) PCR (Polymerase Chain Reaction)
d) Metagenomics Answer: c) PCR (Polymerase Chain Reaction) - What is the primary purpose of a Kirby-Bauer disk diffusion test?
a) Identifying bacterial species
b) Quantifying bacterial growth
c) Assessing antibiotic susceptibility
d) Detecting fungal infections Answer: c) Assessing antibiotic susceptibility - What is the primary advantage of using MALDI-TOF mass spectrometry for microbial identification?
a) It provides information about bacterial motility.
b) It allows for the visualization of bacterial colonies.
c) It provides rapid and accurate identification based on protein profiles.
d) It measures antibiotic sensitivity. Answer: c) It provides rapid and accurate identification based on protein profiles. - Which of the following microscopes is most suitable for studying the internal structures of microorganisms in detail?
a) Scanning electron microscope (SEM)
b) Transmission electron microscope (TEM)
c) Phase-contrast microscope
d) Confocal microscope Answer: b) Transmission electron microscope (TEM) - What does the term “aseptic technique” emphasize in microbiology laboratory practices?
a) Maintaining a sterile environment
b) Rapidly culturing microorganisms
c) Using selective media
d) Identifying microorganisms based on DNA sequencing Answer: a) Maintaining a sterile environment
- Which of the following is NOT a common component of personal protective equipment (PPE) used in microbiology laboratories?
a) Lab coat
b) Safety goggles
c) Hairnet
d) Office attire Answer: d) Office attire - In microbiology, what is the primary function of using selective media?
a) To encourage the growth of all microorganisms
b) To promote rapid bacterial replication
c) To inhibit the growth of specific microorganisms
d) To enhance bacterial motility Answer: c) To inhibit the growth of specific microorganisms - What does the biosafety level (BSL) designation of a laboratory indicate?
a) The laboratory’s location
b) The level of funding provided to the laboratory
c) The types of microorganisms that can be safely handled in the laboratory
d) The laboratory’s cleanliness standards Answer: c) The types of microorganisms that can be safely handled in the laboratory