Tissue processing is an important procedure in the histopathology laboratory. It is used to preserve the anatomy of the tissue. In order to cut the fine section of tissue, we need suitable hardness and consistency in the tissue. Therefore these properties can be imparted by the infiltration, by the paraffin wax, celloidin, and by resins. Some …
Methyl red test or MR test is an important biochemical test used to differentiate the enterobacteria. Methyl red is used as a PH indicator. Methyl red test principle Some bacteria create sufficient acidity from glucose to give RED color with methyl red. While other organisms do not produce more acidity from glucose. Therefore in the …
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CAMP test stands for Christie, Atkins, Munch-Peterson test in microbiology. A clear area around the blood agar plate is the sign of a positive CAMP test. CAMP test principle A positive CAMP test produces a clear area around the colony on the surface of a blood agar plate that has been affected by the staphylococcus …
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The litmus milk decolorization test is an important biochemical; test used to identify the clostridia. Litmus milk test principle A heavy inoculum of the test organism is incubated for up to 4 hours in a tube containing litmus milk. A change indicates a reduction of litmus milk in the medium color from mauve to white …
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The phenylalanine deaminase test is important to differentiate proteus and providendia from other enterobacteria and enterocolitis. Principle of phenylalanine deaminase test Some enterobacteria members can form phenyl pyruvic acid from phenylalanine by oxidative deamination. When this phenylalanine reacts with acidified ammonium sulfate OR 10 % ferric chloride solution to form phenyl pyruvic acid.This phenyl pyruvic …
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Arginine is an amino acid that builds up protein. Arginine is hydrolyzed by some bacteria, which release an enzyme called arginine dihydrolase. It is the characteristics of certain enterobacteria. Arginine dihydrolase test procedure There are two types of procedures. Procedure: 1 Following are the steps in procedure 1. Inoculate 5 ml arginine broth. Incubate for …
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Asculine is a glycoside and it is incorporated with a nutrient base a ferric salt. Aesculin hydrolysis means the breakdown of aesculin. This hydrolysis is indicated by a BROWN coloration. It occurs due to the reaction of aglycone (6.7 Dihydroxycoumarin) with iron. Sometimes another chemical (SODIUM AZIDE) is added. It acts as a preservative. Aesculin …
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The oxidation fermentation test was first discovered by the two scientists Hugh and Leifson in 1953. Therefore oxidation fermentation test was nominated as Hugh and Leifson test. The purpose of the Hugh and Leifson test is to differentiate between those organisms that oxidize carbohydrates ( aerobic organisms) and between those organisms that don’t oxidize carbohydrates …
