Thrombin is an important enzyme in the coagulation pathway. The thrombin time principle is simply that, this enzyme changes the fibrinogen into fibrin in the coagulation pathway. Time spend during clot formation after the addition of Thrombin is called Thrombin time. Thrombin time test Requirement: procedure for the thrombin time What is the Reference Range … Read more
Leishman stain is a commonly used stain in the hematology lab. It is used to identify the white blood cells, malaria parasite, cutaneous leishmania, and Trypanosoma species. Leishman stain preparation Requirements Following are the requirements of Leishman stain preparation. Leishman stain procedure
A platelet count blood test is important for the calculation of platelet in the blood. Like other blood tests Hb, CBC, LFT (liver function test), RFT (Renal function test), and platelet can be counted by two methods. Note: Both methods are used but the manual or visual method is more reliable than an electronic particle … Read more
Mostly white blood cells (WBC) or TLC are calculated by an automated hematology analyzer but WBC can be calculated by the formula for WBC count in the Neubauer chamber (TLC test). WBC / TLC count Principle Anticoagulated blood mixed with diluting fluid in a WBC pipette. After mixing the charge in the Neubauer chamber. Count … Read more
Anemia is defined as a low hemoglobin level. There are three types of anemia. Microcytic hypochromic anemia is one of the most occurring types. Signs, symptoms, and lab diagnosis of microcytic hypochromic anemia is different from other kinds of anemia. Clinical Laboratory finding in microcytic hypochromic anemia Following are the lab finding in microcytic hypochromic … Read more
Anemia is defined as low hemoglobin (HB) concentration or decreased amount of red blood cell mass in the blood. There are three different types of anemia on the basis the of Morphological classification of the anemia chart. Hemoglobin level depends on many factors like gender, age, nutrition, and health. The female has lower Hemoglobin as … Read more
hematoxylin and eosin stain is commonly used stains in the histopathology and cytology section of the clinical laboratory. It is preferred for viewing cellular and tissue structure detail by pathologists. It demonstrates the broad range of cytoplasmic, nuclear, and extracellular matrix features of the tissue. Hematoxylin eosin stain Principle First of all, wax cleared tissues … Read more
Red blood cells (RBC) have a negative charge on their surface due to SIALIC ACID. This charge prevents the clumping of Red blood cells. Zeta Potential principle RBCs have a negative charge on their surface due to sialic acid. Therefore red blood cells repel the adjacent red blood cells (Having the same charge), result in … Read more
The sickling test, also known as the sickle cell test, is a laboratory test used to diagnose sickle cell disease, (a genetic blood disorder) . It involves taking a small sample of blood from the patient and examining it under a microscope to look for the presence of sickle-shaped red blood cells. Therefore Sickling test … Read more
The hematocrit is an important test for estimating the average volume of red blood cells. Anticoagulated blood is used for hematocrit tests. You can calculate mean corpuscular volume (MCV) with the help of HCT. Manual hematocrit calculation methods There are two methods for calculating the hematocrit. Wintrobe’s method of Hematocrit (macrohematocrit) test requirements, procedure , … Read more