How to make 0.9 normal saline solution at home complete Guide

0.9% normal saline solution is a sterile mixture of salt (sodium chloride) and water. It closely matches the salt concentration in human blood.

It is commonly used for medical purposes, like hydration, wound cleaning, and contact lens rinsing.

In this article you will learn about how to prepare a 0.9% normal saline solution safely.

Ingredients and Equipment

To make a 0.9% normal saline solution, you will need the following:

Ingredients

Water – 1 liter (distilled or boiled and cooled)
Salt (Sodium Chloride, NaCl) – 9 grams (non-iodized table salt or sea salt)

Equipment

Measuring cup
Weighing scale (for precise measurement of salt)
Clean glass jar or sterile container
Stirring spoon

Step-by-Step Guide

Step 1: Measure the Water

Start by measuring 1 liter of clean water. If you do not have distilled water, boil tap water for at least 15 minutes. Let it cool to room temperature before using.

Step 2: Weigh the Salt

Weigh exactly 9 grams of salt using a digital kitchen scale. If you don’t have a scale, this is roughly 1.5 teaspoons of salt.

Step 3: Mix the Solution

Pour the 9 grams of salt into the 1 liter of water. Stir the solution thoroughly until the salt is completely dissolved.

Step 4: Store the Solution

Transfer the saline solution into a sterile glass jar or bottle. If you are using it for medical purposes, ensure the container is properly disinfected before pouring.

Read more how to prepare Buffer solution.

Safety and Storage Tips

How to Store

Store the saline solution in a clean, airtight container.
Keep it at room temperature in a cool, dry place.
Label the container with the date of preparation.

When to Discard

Use within 24 hours if not stored in a sterile container.
If stored in a sterile and sealed container, it can last up to one month.
Discard immediately if the solution appears cloudy or contaminated.

Uses of 0.9% Normal Saline Solution

This saline solution has several applications, including:

Medical and Health Uses of 0.9% normal saline

Wound cleaning – Helps flush out debris and prevent infection.
Nasal irrigation – Clears nasal passages from mucus and allergens.
IV hydration (only in clinical settings) – Restores fluid balance.

Home and Personal Care

Contact lens rinse – A temporary alternative if proper lens solution is unavailable.
Skin care – Used for mild cleansing and soothing irritations.