A urine culture and sensitivity test is a crucial diagnostic test used to identify the microorganisms causing urinary tract infections (UTIs).
This test not only confirms the presence of pathogens in the urine but also determines their susceptibility to specific antibiotics.
Urine culture and sensitivity test procedure steps
- Instruct the patient to clean the genital area thoroughly with a cleansing wipe. Use a sterile urine collection cup for sample collection.
- Collect a midstream urine sample to minimize contamination from the urethra or external genitalia.
- Transfer the urine sample to a sterile container suitable for transportation.
- In the laboratory, a small amount of urine is spread onto specific culture media that promote the growth of bacteria and fungi.
- Place the culture plates in an incubator set at the appropriate temperature for the growth of urinary pathogens. Typically 24 to 48 hours, to allow microbial colonies to form.
- Conduct Gram staining and other preliminary tests for identification.
- After incubation, visually examine the culture plates for the presence of microbial colonies.
- Quantify the number of colonies to assess the degree of bacterial or fungal growth.
- NOTE: High colony counts may indicate a significant infection.
- Inoculate the identified microorganisms onto agar plates containing different antibiotics.
- Observe the response to antibiotics to determine the sensitivity or resistance of the pathogens.
- Specify the antibiotics to which the identified pathogens are sensitive or resistant.
- Finally a positive result indicates the presence of an UTI , while a negative result suggests no significant growth.
Urine culture test in hindi
मूत्र संवेदन परीक्षण एक महत्वपूर्ण डायग्नोस्टिक टूल है जिसका उपयोग मूत्र में किसी भी संक्रमण करने वाले सूक्ष्मजीवों की पहचान और विशिष्ट एंटीबायोटिक्स के प्रति उनकी संवेदनशीलता की जानकारी के लिए किया जाता है।
- संक्रमण की पहचान: मूत्र संक्रमण आम हैं और इसका पता उस समय लगाया जा सकता है जब मूत्र में तकलीफदेह अवस्था, पेशाब के दौरान दर्द या मूत्र की गंध या बदबू आती है।
- उपचार के लिए विशिष्टता: मूत्र संवेदन परीक्षण द्वारा सूक्ष्मजीवों की पहचान होने पर विशिष्ट एंटीबायोटिक्स का चयन करने में मदद मिलती है, जिससे उपयुक्त और प्रभावी उपचार किया जा सकता है।
what does a urine culture show
A urine culture reveals the presence of bacteria or other microorganisms in the urine, indicating a potential urinary tract infection (UTI). It helps identify the specific pathogens causing the infection .
urine culture test positive means
A positive urine culture test indicates the presence of bacteria or microorganisms in the urine, suggesting an infection in the urinary tract.
strep b in urine culture not pregnant what it mean
The presence of Group B Streptococcus (Strep B) in a urine culture for a non-pregnant individual may indicate colonization of the bacteria in the urinary tract.
what is the normal colony count in urine culture
The normal colony count in a urine culture is generally considered to be less than 100,000 colony-forming units per milliliter (CFU/mL) for a midstream clean-catch urine sample. This threshold helps differentiate between contamination during sample collection and a true urinary tract infection
what causes mixed flora in urine culture
Mixed flora in urine culture often results from contamination during collection, prolonged storage, or procedures like catheterization. It may also be associated with urinary tract infections (UTIs) and compromised immune function.
why is cled agar used for urine culture
CLED agar (Cystine Lactose Electrolyte Deficient) is used for urine culture because it inhibits the swarming of Proteus species and supports the growth of a variety of urinary pathogens. It helps in isolating and identifying bacteria from urine samples.
what is a reflexive urine culture
A reflexive urine culture is ordered automatically if initial routine urinalysis suggests potential infection, prompting further testing to identify and analyze the specific bacteria causing the infection.
what is a reflex urine culture
A reflex urine culture is an additional test automatically triggered based on the results of an initial urinalysis. If certain parameters in the initial analysis suggest a potential infection, a reflex urine culture is performed to identify and analyze the specific bacteria causing the infection.