Serum amylase test is important for calculation of hydrolytic enzyme which hydrolyses starch into maltose. It present in saliva and pancreatic juice where it is secreted by parotid glands and pancreas respectively. Small amounts of leak into circulation due to where and tear of cells in these glands. The circulating enzyme is excreted by the … Read more
The Schick test is a diagnostic tool used to determine an individual’s immune status against diphtheria toxin. Schick test principle The test involves injecting a small amount of inactivated diphtheria toxin just below the surface of the skin, usually on the forearm. If the individual has sufficient levels of antibodies against the toxin, there will … Read more
cohb blood test used to diagnose carboxyhemoglobin in the blood. Carboxyhemoglobin blood test procedure Normal blood is diluted 1:300 in a dilute ammonia solution (it prevents the precipitation of plasma proteins). It is placed in the cell of the Spectroscope. The instrument is set in such a way that bands of oxyhaemoglobin’s spectra overlap exactly. … Read more
The Bile Tolerance test is used to differentiate streptococcus agalactia from other gram-positive cocci. Certain streptococci (S.agalactiae) and enterococci are resistant to 10-40% bile and this test is used to differentiate these from other Gram-positive cocci. Anaerobic bacteria also vary in their ability to grow in the presence of 20% bile. Bile tolerance is most … Read more
The potassium cyanide medium test (KCN test) is a microbiological test used to identify bacteria that can grow in the presence of potassium cyanide. The test is based on the fact that some bacteria are capable of utilizing KCN as a sole source of nitrogen. Potassium cyanide medium test Reagent (KCN test) Following are the … Read more
Voges Proskauer test or VP test is used to identify some enterobacteria. But some kinds of it are VP negative. The optimal temperature for the VP test is 37 C but some groups show negative results at this temperature. The Hafina group shows negative results at 37 C, but it gives positive results at 30 … Read more
Methyl red test or MR test is an important biochemical test used to differentiate enterobacteria. Methyl red is used as a PH indicator. Methyl red test principle Some bacteria create sufficient acidity from glucose to give RED color with methyl red. While other organisms do not produce more acidity from glucose. Therefore in the negative … Read more
CAMP test stands for Christie, Atkins, Munch-Peterson test in microbiology. A clear area around the blood agar plate is a sign of a positive CAMP test. CAMP test principle A positive CAMP test produces a clear area around the colony on the surface of a blood agar plate that has been affected by the staphylococcus … Read more
The litmus milk decolorization test is an important biochemical; test used to identify clostridia. Litmus milk test principle A heavy inoculum of the test organism is incubated for up to 4 hours in a tube containing litmus milk. A change indicates a reduction of litmus milk in the medium color from mauve to white or … Read more
The phenylalanine deaminase test is important to differentiate proteus and providendia from other enterobacteria and enterocolitis. Principle of phenylalanine deaminase test Some enterobacteria members can form phenyl pyruvic acid from phenylalanine by oxidative deamination. When this phenylalanine reacts with acidified ammonium sulfate OR 10 % ferric chloride solution to form phenyl pyruvic acid.This phenyl pyruvic … Read more