Here’s the Giemsa stain procedure, composition, preparation steps.

Giemsa stain is a histological and cytological stain commonly used in laboratories to enhance the visibility of components, especially in blood smears and bone marrow samples.

Giemsa stain principle

The Giemsa stain use mixture of dyes to selectively stain cellular components, aiding in the visualization and classification of blood cells and other cell types in specimens.

Preparation of giemsa stain

1. Dissolve Giemsa powder in methanol to create a stock solution.
2. Dilute the stock solution with buffered water to achieve the desired concentration.
3. Apply the prepared Giemsa stain to a fixed and dried specimen.
4. Allow the stain to interact with the specimen for a specified duration.
5. Rinse the specimen to remove excess stain.
6. Air-dry the specimen before examination under a microscope.

Giemsa stain composition

The composition of Giemsa stain are:

• Methylene Blue
• Eosin
• Azure B
• Glycerol or methanol (as a solvent)

Giemsa stain procedure

1. Dissolve Giemsa powder in methanol to create a stock solution.
2. Dilute the stock solution with buffered water to achieve the desired concentration.
3. Apply the Giemsa stain to a fixed and air-dried histological or cytological specimen.
4. Allow the Giemsa stain to interact with the specimen for a specified duration.
5. Rinse the specimen with buffered water to remove excess Giemsa stain.
6. Allow the specimen to air-dry thoroughly.
7. Finally Examine the stained specimen under a microscope, observing the differential coloration of cellular structures.
8. Record and analyze the observed features or abnormalities in the stained specimen.