Tissue Processing Steps, Dehydration, clearing, impregnation, embedding histopathology lab

Tissue processing is an important procedure in the histopathology laboratory. It is used to preserve the anatomy of the tissue. In order to cut the fine section of tissue, we need suitable hardness and consistency in the tissue. Therefore these properties can be imparted by the infiltration, by the paraffin wax, celloidin, and by resins. Some …

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Phenylalanine deaminase test principle, procedure, and purpose

The phenylalanine deaminase test is important to differentiate proteus and providendia from other enterobacteria and enterocolitis. Principle of phenylalanine deaminase test Some enterobacteria members can form phenyl pyruvic acid from phenylalanine by oxidative deamination. When this phenylalanine reacts with acidified ammonium sulfate OR 10 % ferric chloride solution to form phenyl pyruvic acid.This phenyl pyruvic …

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Arginine dihydrolase test procedure for pseudomonas aeruginosa

Arginine is an amino acid that builds up protein. Arginine is hydrolyzed by some bacteria, which release an enzyme called arginine dihydrolase. It is the characteristics of certain enterobacteria. Arginine dihydrolase test procedure There are two types of procedures. Procedure: 1 Following are the steps in procedure 1. Inoculate 5 ml arginine broth. Incubate for …

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Oxidation fermentation test(Hugh and Leifson test ) principle, the procedure for P.Aeruginosa

The oxidation fermentation test was first discovered by the two scientists Hugh and Leifson in 1953. Therefore oxidation fermentation test was nominated as Hugh and Leifson test. The purpose of the Hugh and Leifson test is to differentiate between those organisms that oxidize carbohydrates ( aerobic organisms) and between those organisms that don’t oxidize carbohydrates …

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