Clinical Microbiology test: H&E stain, Gram stain, Culture Media…
The oxidation fermentation test was first discovered by the two scientists Hugh and Leifson in 1953. Therefore oxidation fermentation test was nominated as Hugh and Leifson test. The purpose of the Hugh and Leifson test is to differentiate between those organisms that oxidize carbohydrates ( aerobic organisms) and between those organisms that don’t oxidize carbohydrates … Read more
India ink stain is used for visualizing the capsule of encapsulated B. anthracis and other capsulated organisms, especially the fungus Cryptococcus neoformans. Cryptococcus neoformans are identified from blood, blood culture bottles, or cerebrospinal fluid (CSF). Material for India ink stain procedure Following are the materials for the procedure. India ink stain for cryptococcus procedure India … Read more
Heat fixation is the second method after the chemical fixation in histology. what is heat fixation in microbiology? The mechanism of heat fixation is, that it denatures the protein. It takes two to three minutes. Microwave ovens have overtaken the conventional heating method, due to their controlled environment. 45-55 C is the optimum temperature of … Read more
Here is the most important Quizlet of the microbiology final exam for medical students to refresh their knowledge. Chose the gram-positive bacteria. a. Neisseria, b. Klebsiella, c. Clostridium Free-living, Thick wall, cause abscess of skin. Characteristics of. a. Streptococcus b. Staphylococcus c. Bacillus Extracellular bacteria, thick wall, spore-forming aerobe Characteristics of. a. Clostridium b. Corynebacterium … Read more
There are many differences between gram-positive and gram-negative bacteria. There are many differences between gram-positive and negative bacteria cell membranes the base of the cell wall, cell wall compositions, gram stains, antibiotics, and their toxins(endotoxin, exotoxins). Here we will elaborate on the difference between gram-positive and gram-negative bacteria is the base of gram stain. Gram … Read more
When you’re seeking to conceive or freezing sperm for later, you need all conditions to be just right. So you get healthy, improve your daily diet, and cut out the toxins. But can specific “superfoods for male fertility” actually change the quality of your sperm? Researches show that what you eat can grow sperm quality, … Read more
The nitrate reduction test is an important biochemical test in microbiology. It is used to differentiate the various gram-negative rods and also mycobacteria. Nitrate reduction test principle Some anaerobic bacteria use nitrates in an anaerobic environment. As a result, they produce NITRITE and NITROGEN gas. Nitrite changes the color of media, while nitrogen gas can … Read more
H2S test is an important test in microbiology for the identification of various enterobacteria and Brucella species. H2S test principle H2S (hydrogen sulfide) produced by many bacteria due to reaction with sulphur containing amino-acid. This H2S (hydrogen sulfide) detected by a change in colour by the reaction of H2S and ferrous chloride leading to black … Read more
The Nagler reaction test is used to identify the gram-positive bacilli clostridium perfringens. Clostridium perfringens have six serotypes from A to F, but only three A, C, and F types cause disease. Clostridium perfringen causes gas gangrene and food poisioning. Nagler reaction test principle Clostridium per fringe bacteria produces lecithinase, which creates opalescence in the … Read more
Mantoux skin test or PPD skin test is used to diagnose tuberculosis (TB). It is used to check the hypersensitivity of an individual to tb. Mantoux skin test procedure (ppd 2-step procedure) There are various doses of tuberculin like 1 TU, 5 TU, 10 TU, and 250 TU. But mostly 5 TU is used. Mantoux … Read more