A microtome is a laboratory instrument used for cutting extremely thin sections of materials, typically biological specimens, for microscopic examination. It is an essential tool in various scientific fields, including biology, and histopathology. These instruments are essential for studying the intricate structures of tissues, cells, and various specimens, contributing significantly to our understanding of various … Read more
Gout is a type of arthritis that affects millions of people worldwide. It is characterized by sudden and severe pain, redness, and swelling in the joints, particularly in the big toe. This painful condition is caused by the buildup of uric acid crystals in the joints, which can result in an inflammatory response. Gout attacks … Read more
Following are the properties of ideal fixatives (buffered formalin) Classification of chemical fixatives in the Histopathology laboratory Fixatives are classified into three groups. What are the tissue fixative examples? These fixatives are specially used for tissue fixation. Following fixatives used for tissue What are Histochemical fixative names? These fixatives are specially used for histochemical fixation. … Read more
It is fixation is important in tissue processing because it preserves the anatomy of different tissues. It makes tissue hard so that a thin section can be made from it. Tissue fixation means the process by which components of the cell and tissues are fixed physically and partially by chemical methods. So that it … Read more
There are many types of specimens for cytological examination. Cytology specimen collection and handling are very sensitive. Each sample has its own standard operating procedure. But some steps are common in all. We need these things Fixatives Container or jar – Coplin jar made of glass or plastic is used as a fixative … Read more
We collect and deal with different types of tissues biopsy in the histopathology laboratory. The tissue collection method in the histology laboratory has the following steps. Fixatives Container Request Form Labeling of specimen Role of Fixative in tissue collection method After the removal of surgical specimens, they should be placed in a container filled with … Read more
Tissue staining is the second step after collection.Different tissues are colored.differently.There are three main types of histology stains. Specially prepared dyes are used for such staining purposes. Such dyes are prepared by adding the cytochrome to a chromophore. Note: Cytochrome is a compound when mixed with chromophore, forms a dye. This dye can be acidic … Read more
Decalcification is a process used to remove calcium salts from tissue samples, primarily in histology and pathology laboratories, to make them suitable for further processing and microscopic examination. There are many methods, which used to check the decalcification of the different types of tissues. Here we will learn about “End point of decalcification in histopathology”. … Read more
Tissue processing is an important procedure in the histopathology laboratory. It is used to preserve the anatomy of the tissue. In order to cut the fine section of tissue, we need suitable hardness and consistency in the tissue. Therefore these properties can be imparted by the infiltration, by the paraffin wax, celloidin, and by resins. Some … Read more
hematoxylin and eosin stain is commonly used stains in the histopathology and cytology section of the clinical laboratory. It is preferred for viewing cellular and tissue structure detail by pathologists. It demonstrates the broad range of cytoplasmic, nuclear, and extracellular matrix features of the tissue. Hematoxylin eosin stain Principle First of all, wax cleared tissues … Read more