Histopathology

Properties of 10 neutral buffered formalin ideal fixative histopathology

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Properties of an ideal fixative in histopathology

Following are the properties of ideal fixatives (buffered formalin) Classification of chemical fixatives in the Histopathology laboratory Fixatives are classified into three groups. What are the tissue fixatives examples?  These fixatives are specially used for tissue fixation. Following fixatives used for tissue What are Histochemical fixatives names? These fixatives are specially used for histochemical fixation. … Read more

Tissue fixation types mechanism( bone, skin, biopsies, liver) histology

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It is fixation is important in tissue processing because it preserves the anatomy of different tissues. It makes tissue hard so that a thin section can be made from it. Tissue fixation means the process by which components of the cell and tissues are fixed physically and partially by chemical methods. So that it can … Read more

Cytology specimen collection and handling (Sputum specimen) in histology lab

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There are many types of specimens for cytological examination. Cytology specimen collection and handling are very sensitive. Each sample has its own standard operating procedure. But some steps are common in all.  We need these things Fixatives Container or jar – Coplin jar made of glass or plastic is used as a fixative container. Request … Read more

tissue collection method in histology laboratory (Liver, bone, renal)

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Tissue collection method histology

We collect and deal with different types of tissues biopsy in the histopathology laboratory. The tissue collection method in the histology laboratory has the following steps. Fixatives Container Request Form Labeling of specimen Role of Fixative in tissue collection method After the removal of surgical specimens, they should be placed in a container filled with … Read more

Types of Histology stains – acidic, basic, neutral stains

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3 main types of histology stain

Tissue staining is the second step after collection.Different tissues are colored.differently.There are three main types of histology stains. Specially prepared dyes are used for such staining purposes. Such dyes are prepared by adding the cytochrome to a chromophore. Note: Cytochrome is a compound when mixed with chromophore, forms a dye. This dye can be acidic … Read more

Chemical method of endpoint decalcification procedure histopathology techniques

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There are many methods, which used to check the decalcification of the bones. But the most commonly used method is end-point decalcification.  It is used to check the calcium concentration in the sample (Bone). Endpoint Decalcification chemical test Principle Decalcified tissue (bone) is placed in the decalcifying agents. If tissue is partially calcified then a … Read more

Tissue Processing Steps, Dehydration, clearing, impregnation, embedding histopathology lab

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Tissue processing is an important procedure in the histopathology laboratory. It is used to preserve the anatomy of the tissue. In order to cut the fine section of tissue, we need suitable hardness and consistency in the tissue. Therefore these properties can be imparted by the infiltration, by the paraffin wax, celloidin, and by resins. Some … Read more

Hematoxylin-eosin staining Principle, Procedure, protocol (H&E stain)

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hematoxylin and eosin stain is commonly used stains in the histopathology and cytology section of the clinical laboratory. It is preferred for viewing cellular and tissue structure detail by pathologists. It demonstrates the broad range of cytoplasmic, nuclear, and extracellular matrix features of the tissue. Hematoxylin eosin stain Principle First of all, wax cleared tissues … Read more