Cytology vs histology

Here you will learn about Cytology vs histology. Cytology is widely used in diagnostic medicine, especially in the identification of abnormal cells indicative of diseases such as cancer. Fine needle aspiration and pap smear are the most common techniques in cytology. On the other side, Histology unveils tissue intricacies, showcasing cell collaboration and specialized functions. … Read more

How to use microtome, types of microtome

how to operate microtome machine types

A microtome is a laboratory instrument used for cutting extremely thin sections of materials, typically biological specimens, for microscopic examination. It is an essential tool in various scientific fields, including biology, and histopathology. These instruments are essential for studying the intricate structures of tissues, cells, and various specimens, contributing significantly to our understanding of various … Read more

What causes high uric acid levels in the body

close up shot of a wounded knee

Gout is a type of arthritis that affects millions of people worldwide. It is characterized by sudden and severe pain, redness, and swelling in the joints, particularly in the big toe. This painful condition is caused by the buildup of uric acid crystals in the joints, which can result in an inflammatory response. Gout attacks … Read more

Properties of 10 % neutral buffered formalin fixative histopathology

Following are the properties of ideal fixatives (buffered formalin) Classification of chemical fixatives in the Histopathology laboratory Fixatives are classified into three groups. What are the tissue fixative examples?  These fixatives are specially used for tissue fixation. Following fixatives used for tissue What are Histochemical fixative names? These fixatives are specially used for histochemical fixation. … Read more

Tissue fixation types mechanism( bone, skin, biopsies, liver) histology

It is fixation is important in tissue processing because it preserves the anatomy of different tissues. It makes tissue hard so that a thin section can be made from it.   Tissue fixation means the process by which components of the cell and tissues are fixed physically and partially by chemical methods. So that it … Read more

Cytology specimen collection and handling (Sputum specimen) in histology lab

There are many types of specimens for cytological examination. Cytology specimen collection and handling are very sensitive. Each sample has its own standard operating procedure. But some steps are common in all.  We need these things     Fixatives Container or jar – Coplin jar made of glass or plastic is used as a fixative … Read more

Tissue collection method in histology laboratory (Liver, bone, renal)

We collect and deal with different types of tissues biopsy in the histopathology laboratory. The tissue collection method in the histology laboratory has the following steps. Fixatives Container Request Form Labeling of specimen Role of Fixative in tissue collection method After the removal of surgical specimens, they should be placed in a container filled with … Read more

Types of Histology stains – acidic, basic, neutral stains

Tissue staining is the second step after collection.Different tissues are colored.differently.There are three main types of histology stains. Specially prepared dyes are used for such staining purposes. Such dyes are prepared by adding the cytochrome to a chromophore. Note: Cytochrome is a compound when mixed with chromophore, forms a dye. This dye can be acidic … Read more

End point of decalcification in histopathology procedure (physical method, chemical method)

End point of decalcification in histopathology procedure techniques

Decalcification is a process used to remove calcium salts from tissue samples, primarily in histology and pathology laboratories, to make them suitable for further processing and microscopic examination.  There are many methods, which used to check the decalcification of the different types of tissues. Here we will learn about “End point of decalcification in histopathology”.  … Read more

Tissue Processing Steps, Dehydration, clearing, impregnation, embedding histopathology lab

Tissue processing is an important procedure in the histopathology laboratory. It is used to preserve the anatomy of the tissue. In order to cut the fine section of tissue, we need suitable hardness and consistency in the tissue. Therefore these properties can be imparted by the infiltration, by the paraffin wax, celloidin, and by resins. Some … Read more