Histopathology

10% neutral buffered formalin ideal fixative properties in histopathology

Properties of an ideal fixative in histopathology

Following are the properties of ideal fixatives (buffered formalin) An ideal fixative prevents autolysis and bacterial decomposition in the tissue. It preserves the tissue in its natural state. Fix all tissue components at their position. Makes the cellular component insoluble to the reagent used in tissue processing.  Preserve the tissue volume. Avoid excessive hardness of …

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Cytology specimen collection and handling (Sputum specimen) in histology lab

There are many types of specimens for cytological examination. Cytology specimen collection and handling are very sensitive. Each sample has its own standard operating procedure. But some steps are common in all.  We need these things Fixatives Container or jar – Coplin jar made of glass or plastic is used as a fixative container. Request …

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tissue collection method in histology laboratory (Liver, bone, renal)

Tissue collection method histology

We collect and deal with different types of tissues biopsy in the histopathology laboratory. The tissue collection method in the histology laboratory has the following steps. Fixatives Container Request Form Labeling of specimen Role of Fixative in tissue collection method After the removal of surgical specimens, they should be placed in a container filled with …

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Types of Histology stains – acidic, basic, neutral stains

3 main types of histology stain

Tissue staining is the second step after collection.Different tissues are colored.differently.There are three main types of histology stains. Specially prepared dyes are used for such staining purposes. Such dyes are prepared by adding the cytochrome to a chromophore. Note: Cytochrome is a compound when mixed with chromophore, forms a dye. This dye can be acidic …

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Chemical method of endpoint decalcification procedure histopathology techniques

There are many methods, which used to check the decalcification of the bones. But the most commonly used method is end-point decalcification.  It is used to check the calcium concentration in the sample (Bone). Endpoint Decalcification chemical test Principle Decalcified tissue (bone) is placed in the decalcifying agents. If tissue is partially calcified then a …

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Tissue Processing Steps, Dehydration, clearing, impregnation, embedding histopathology lab

Tissue processing is an important procedure in the histopathology laboratory. It is used to preserve the anatomy of the tissue. In order to cut the fine section of tissue, we need suitable hardness and consistency in the tissue. Therefore these properties can be imparted by the infiltration, by the paraffin wax, celloidin, and by resins. Some …

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