Here you will learn “Sahli’s Hemoglobin measurement methods, and procedure”.

Hemoglobin (Hb) is an important content of our blood.  It exists in the red blood cells. There are two hemoglobin measurement methods. Here is Sahli’s method of hb estimation.

Hb test by Sahli method procedure (sahli’s hb method)

Hemoglobin is an oxygen-carrying protein in the blood. It carries oxygen from the lungs and transports it to different parts of the body like the heart, brain, kidney, liver, hand, nose, ear, and others. It has iron in the chain. Hemoglobin has two pairs of chains. One pair is called the Alpha chain while the second pair is the Beta chain. Both chains interlink with disulfide bridges.

Explain the method of testing for hemoglobin using Sahli’s haemometer

To estimate Hb by Sahli’s method is easy, we convert hemoglobin into Hematin by adding 0.1 N Hydrochloric Acid. The resultant compares with the reference colored solution or color strip. Following are the steps.

Note: In Sahli’s method, the hemoglobin meter should be kept on a level surface.

  •  Fill the tube with HCl up to 2 marks.
  • Place the tube in the Hemoglobin meter
  • Suck the blood in Sahli’s pipet up to 20 marks, and drop this blood into the graduated tube. as explained in the video.
  • Mix the solution with the help of a stirrer and wait for 3-5 minutes.
  • After this put the drops of saline one by dropper in the graduate tube, while doing this compare the color of the solution with the Hemocytometer reference colored strip.
  • NOTE the reading on the graduated tube. This is the Hb amount in the blood.

Sahli’s Hb test requirements

  • Sahli’s haemometer
  • Sahli’s pipette
  • 0.1 N HCL
  • Dropping Pipette

Hemoglobin normal range female

The hemoglobin range for females is 12 to 16. While the hemoglobin range for males is 13 to 17 mg/dl. This difference in hemoglobin is due to hormonal differences or menses cycles in females.

Hemoglobin normal ranges in males, female

The hb normal range for males and females 

  • Male            13-17 mg/dl
  • Fe-male       12-16 mg/d

Here’s the video of “Sahli’s hemoglobin measurement methods”.

  1. what method is used to determine the hemoglobin level

    Sahli’s Hemoglobin measurement method is used to calculate the hemoglobin level.

  2. How accurate is Sahli’s method?

    Sahli’s method is less accurate than other methods (such as the cyanmethemoglobin method) and may be affected by variations in the dilution of the blood sample or the timing of the measurement.
    However, it remains a useful method for routine screening of hemoglobin levels.

  3. What are the advantages of Sahli’s method?

    Sahli’s method is simple, inexpensive, and widely available, making it a useful tool for routine screening of hemoglobin levels.

  4. What are the limitations of Sahli’s method?

    1. Sahli’s method is less accurate than other methods and may be affected by variations in the dilution of the blood sample or the timing of the measurement.
    2. It also requires some training and experience to perform correctly.
    3. Sahli’s method may be less suitable for measuring very low or very high levels of hemoglobin.

  5. How long does it take to perform Sahli’s method?

    Sahli’s method is a relatively quick procedure that usually takes a few minutes to perform.

  6. What equipment is needed for Sahli’s method?

    The equipment needed for Sahli’s method includes a
    1. Sahli hemoglobinometer (a device used to measure the hemoglobin concentration),
    2. A pipette (to measure the blood and diluting solution),
    3. A series of color standards (for visual comparison of the red color).

  7. Can Sahli’s method be used to diagnose anemia?

    Yes, Sahli’s method can be used to screen for anemia by measuring the hemoglobin concentration in the blood. However, it should be used in conjunction with other diagnostic tests, such as a complete blood count (CBC), to confirm the diagnosis of anemia and determine its cause.

  8. Are there any risks or side effects associated with Sahli’s method?

    Sahli’s method is generally considered safe and does not pose any significant risks or side effects. However, as with any blood test, there is a small risk of infection or bleeding at the site where the blood sample is taken.

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